For peptide researchers working with doses of 100 to 500 mcg, where the injection volume might be 0.04 to 0.2 mL, this accuracy difference is not trivial
FENIX assays Liposomes were prepared from egg phosphatidylcholine (egg PC, Sigma-Aldrich) in pH 7.4 TBS buffer (25 mM, extruded to 100 nm, Chelex-100 treated) according to our previous report 25,43
The half-maximal effective concentration of vitamin K (EC 50 ) was determined using GraphPad software
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