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Laboratory investigations demonstrate that GHK-Cu exhibits a copper binding affinity (log K = 16.1) that positions it perfectly within the physiological copper transport hierarchystronger than albumin but weaker than ceruloplasminenabling controlled copper transfer to cellular targets

The membranes were then incubated in 1% bovine serum albumin (BSA) solution with the following primary antibodies overnight at 4C: anti-AQP4 (Abcam, ab18956), anti-MMP2 (Abcam, ab37150), anti-MMP9 (Abcam, ab38898), anti-TIMP1 (Abcam, ab109125) and anti-beta actin (Abcam, ab8227)

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