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Biochem Pharmacol 52:11471154 Vichot AA, Rastegar A (2014) Use of anion gap in the evaluation of a patient with metabolic acidosis

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The cleared lysate was incubated with Glutathione Sepharose 4B (17075601, GE Healthcare) for 2 h at 4 C then washed

RanGTP interacts with PlexinB1 and PlexinB2 Immunoprecipitation experiments were next performed to establish whether PlexinB1 and B2 regulate RanGTP levels by direct physical interaction with Ran