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Total RNA was isolated from cells using the miRNeasy Mini Kit (Qiagen, Hilden, Germany), according to manufacturer instructions

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Once inflammation and oxidative stress start building up and superseding the amount of available antioxidants like glutathione, they can cause damage to cells and the mitochondria inside of cells leading to memory loss, anxiety, depression, joint pain, fatigue, weakness, weight gain, and increased risk of diabetes, heart disease, and cancer