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Importantly, supplement with FAD prevented cell death caused by FADS knockdown (Fig

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The SUM159PT cell line (provided by Philippe Benaroch, Cellular transport and immunity team, Curie Institute, Paris, France) was maintained in Hams F12 medium supplemented with 5% heat inactivated FBS, 10 mM HEPES, 1 g/mL hydrocortisone, and 5 g/mL of human insulin

10.1186/scrt474 Stem Cell Res