Lysates were clarified by centrifugation at 27,500 g for 65 min at 4C and subsequent 0.45 m filtration
With potassium and sodium channels blocked, long depolarization pulses induced strong calcium signals that did not attenuate with distance, indicating that voltage-gated calcium channels in these cells could support calcium influx throughout the dendritic tree (Goldberg et al., 2003a)
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These findings highlight the therapeutic potential of DSIP-CBBBP fusion peptide with the most pronounced effect
