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Another study in a canine LA-PV preparation used optical mapping and strong Ca 2+ -loading pharmacological intervention with combined ryanodine (to block normal SR Ca 2+ -release) and isoproterenol (to stimulate cellular Ca 2+ entry/RyR2 hyperphosphorylation), to show reentry and spontaneous activity involving the PVs (49)

Classification of smoking status Smoking status was investigated using the following structured questionnaire: Have you smoked at least 100 cigarettes in your entire life?

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