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Samples were excited using a 40-MHz pulse tuned to 488 nm for both intensity and fluorescence lifetime measurements
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Cells were first washed with KHM buffer (110 mM potassium acetate, 20 mM HEPES, 2 mM MgCl2) for 2 min at 6 psi (41.4 kPa), then treated with 20 M of digitonin for 3 min at 6 psi, and with 50 g/ml proteinase K at 6 psi
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