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In brief, 2 10 3 cells were seeded in each well of a 96-well plate for 4 h, then GHK was added and this time is known as 0 h, and the cells were cultured for 24 and 48 h for detecting cell proliferation

Copper levels typically normalize within 4 to 6 weeks of cessation given the peptide's short half-life and the liver's capacity to excrete copper via bile [4]

Summary of key findings: In vitro: reduced oxidative stress, improved cell function

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