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Enzyme-Linked Immunosorbent Assay (ELISA): A biochemical technique used to detect the presence of nicotine or its metabolites in biological samples

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However, smoking prevalence and associated ill health continued to rise in the developed world in the first three decades following Richard Doll's discovery, with governments sometimes reluctant to curtail a habit seen as popular as a result and increasingly organised disinformation efforts by the tobacco industry and their proxies