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For each gradient, about 30 fractions (400 l) were collected from the top and the radioactivity was quantified by liquid scintillation counting (Perkin Elmer)

In line with the hypothesis that CRSR produces an effect on the ECM, another independent study reported a reduced expression of fibroblast activation protein, which is a collagen component of the ECM and TGF-b ( Future Prospect At present, although conventional pharmacological agents used for uterine fibroid continue to exhibit numerous drawbacks, it is still regarded as the ultimate choice of treatment for patients who prefer a noninvasive approach

Geng S, Liu Y, Ma H, et al

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In short, the slides were deparaffinized, rehydrated, and immersed in saturated picric acid solution for 15 minutes and then in Picrosirius for 20 minutes