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Materials and methods Cell culture Colorectal cancer cell lines (RKO, HCT116, DLD1, LOVO, SW620 and CT26), hepatocellular carcinoma cell lines (Hepa 1-6 and HCCLM3), gastric cancer cell line HGC-27, gallbladder cancer cell line GBC-SD and normal colonic epithelial cell line NCM460 were purchased from the Shanghai Institute of Cell Biology, Chinese Academy of Sciences (Shanghai, China)

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Several MMPs play a role in cancer progression, migration, invasion, metastasis and angiogenesis (48)