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Hepatocytes were maintained in RPMI-1640 media supplemented with heat-inactivated 10% fetal bovine serum (FBS) and 1% of 10,000 units penicillin, 10 mg streptomycin and 25 g amphotericin-B at 37C in a 5% CO 2 -95% air incubator (Thermo-forma) with controlled humidity

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For wellness professionals, it may eventually become part of a broader longevity toolkit, pending proper validation