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Indian J Exp Biol 40:12404, PMID:13677625 Morris G, Maes M (2013) Myalgic encephalomyelitis/chronic fatigue syndrome and encephalomyelitis disseminata/multiple sclerosis show remarkable levels of similarity in phenomenology and neuroimmune characteristics

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Blood 86(10):754a, 1995

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For lysosomal enzyme assays, 600 g of whole cell lysates prepared from either WT HEK293T cells, or from untransfected or 48 h transfected LYSET knockout, GNPT knockout, or double knockout cells were incubated with equivalent amounts of the CI-MPR affinity beads for 2 h at 4 C, washed twice with cold Tris-buffered saline containing 1% Tx-100 (pH 7.4) (TBST), then assayed for bound lysosomal enzymes