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The minimum size would be a standard 55-gallon aquarium, but, as always, the bigger the better

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The protective effect of melatonin and epithalon on hypothalamic regulation of the reproductive function in female rats in a model of its premature aging and on the estrous cycles of aging animals in different lighting conditions

Jurado-Flores et al., 2021) and to S-nitrosylation of Cys119 which is adjacent to an Asp residue (Asp118), a combination that is particularly suitable for S-nitrosylation (Hu et al., 2015)