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Therefore, to functionally assess the cells ability to foster lipid biogenesis, we pulsed 67NR, 4T07, and 4T1 cells with 14 C-Glucose and other potential citrate and/or acetyl-CoA precursors (i.e., 14 C-Lactate, 14 C-Acetate, and 14 C-Glutamine) before recovering the radiolabeled lipid fraction

While the morning is preferred for defense, the nighttime window has its own set of advantages centered around repair and recovery

After 50 d of storage, no emulsion breakage, flocculation, or precipitation was observed

Destruction of the methylated, parental DNA plasmid by DpnI digestion followed by E.coli cells transformation allowed the introduction of the desired mutations

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