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Chromatographic separation has been achieved using a Merck RP-Select B HPLC column (105 mm 4.6 mm, 5 m) with a gradient system consisting of 10 mmol/L ammonium acetate (pH 5.5) (A), and methanol (B) at a flow rate of 1 mL/min

Chronic exposure to nicotine, even in its synthetic form, can lead to several liver-related issues: Increased Liver Enzymes Regular use of nicotine products can elevate liver enzymes, which are indicators of liver stress or damage

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