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The extracted DNA is suitable for PCR analyses (Fig
A linearized DNA template was transcribed using T7 RNA polymerase according to standard in vitro transcription conditions
2 mM dithiothreitol
The QuickExtract Plant method allows for the inexpensive processing of one to hundreds of samples simultaneously
YES: without IPTG induction
E. cloni-10G ELITE Electrocompetent Cells (SOLOs) size:12 rxns The extracted DNA is suitableE. cloni 10G and 10GF' Electrocompetent Cells Direct replacements for standard cloning strains (e. g., DH5, DH10B, JM109, TOP10, XL1 Blue, etc.) Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue white screening capable. Available in a range of high transformation efficiencies (5 109 to 4 1010 cfu g). Convenient packaging options. Lucigens E. cloni competent cells share the most useful genetic elements