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cDNAs encoding spinach stromal and thylakoid bound ascorbate peroxidase, differing in the presence or absence of their 3'-coding regions
Arul et al
After washing three times with PBS, cells were fixed with 1 mL 4% paraformaldehyde for 15 min, permeabilized with 1 mL 0.1% Triton X-100 for 10 min at room temperature, and blocked with 1 mL 5% BSA for 30 min