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D-Nle-Tyr-Ile-NH(CH 2 ) 5 CONH 2 eluted at 5.06 min and the internal standard at 4.31 min

Most protocols recommend morning administration on an empty stomach or evening administration at least two hours after dinner

The diagnosis of vitamin B12 deficiency typically relies on low total B12 levels and elevated concentrations of homocysteine (Hcy), as well as levels of transcobalamin-bound B12 (holoTC) and methylmalonic acid (MMA), which serve as functional indicators of cobalamin insufficiency (Carmel and Sarrai, 2006)

It is a comparatively recent discovery and a fast-growing subject of metabolic and longevity research