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Kim MS, Yang HJ, Kim SH, Lee HW, Lee MS

The culture was grown for an additional 16 h at 30 C prior to harvesting by centrifugation at 5000 g for 30 min at 4 C

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Real-time PCR Total RNA was isolated from the cells using the Roche High Pure RNA isolation kit (Roche Life Science, 11828665001) and reverse transcription was performed using the iScript cDNA synthesis kit (Bio-Rad, 1708891) according to the manufacturers instructions

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