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Although the SPAAC DBCO-azide reaction is relatively slow, it proceeds several orders of magnitude faster than the competing glutathione-mediated reduction of azides in biological systems, making this competing process less disruptive to the overall fluorescence readout in our CHAMP assays

doi: 10.1111/j.1365-2184.1994.tb01471.x 65 JenuweinT.AllisC