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NAC provides a precursor to glutathione, while vitamin B6 as P5P supports enzymatic activity involved in normal detoxification and cellular metabolism

By interacting with the tyrosinase enzyme, glutathione can help slow down the overproduction of dark pigment

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This is achieved through the formation of mitochondrial and endoplasmic reticulum contact sites (Oanh et al., 2017)

Alternatively, glutathione can be quantified after reduction of GSSG in the sample with dithiotreitol (DTT) and subsequent derivatization of GSH with mbrB or mchlB via a reverse-phase high-performance liquid chromatography (HPLC) system equipped with a fluorescent or UV detector, or spectrophotometrically after a GR-mediated glutathione recycling assay (total glutathione) from cell organelles after isolation or fractionation