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NAPQI-alkylated peptides found by database searching were confirmed by using the following criteria: 1) precursor mass error 95%, 3) the presence of one diagnostic production (see Figure 3) (Sleno et al., Table 3)

Many practitioners suggest using both since NAD+ increases mitochondrial activity and produces more reactive oxygen species, while glutathione neutralizes these byproducts

However, they often show direct RS scavenging or other biological activity (e.g., inhibition of topoisomerase II)

Differences between groups were statistically analyzed using one-way analysis of variance and Tukey's post-hoc test

Therefore, detecting GSH is crucial for diagnosing cancer and monitoring the redox balance inside the tumor microenvironment (TME) [88]