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The cells were incubated overnight at 4 C with one of the following primary antibodies: rabbit anti-SMAD2 (Cell Signaling Technology, Danvers, MA, RRID: AB_823638, 1:200 dilution), rabbit anti-SMAD3 (Cell Signaling Technology, RRID: AB_2193182, 1:200 dilution), rabbit anti-phospho-SMAD2 (Abcam, RRID: AB_2732791, 1:100 dilution), or rabbit anti-phospho-SMAD3 (Cell Signaling Technology, RRID: AB_2193207, 1:100 dilution)

Journal of Biomaterials Science, Polymer Edition, 2008, 19(8): 969-988

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Author contributions SA: Methodology, Writing original draft

We use the model to study the extent of liver damage caused by overdoses or by chronic use of therapeutic doses, and the effects of polymorphisms in glucoronidation enzymes