Documentation: Keep a daily log of dose, injection site, and any observed effects to maintain protocol consistency and identify tolerance patterns
It protects cells from free radical damage, supports liver detoxification, boosts immunity, and helps regulate melanin production for brighter skin
Blots were blocked in either 5% (w/v) nonfat milk (Lab Scientific, M-0841) or 1% (w/v) BSA in 1 Tris-buffered saline with Tween (TBST) (Teknova, T1688) for 3060 min at room temperature, incubated with primary antibodies in TBST for 1 h at room temperature, washed three times with TBST for 5 min each, incubated with fluorophore-conjugated secondary antibodies for 30 min at room temperature, washed three times with TBST and imaged on an Odyssey CLx imager (LI-COR) or ChemiDoc XRS+ imager with Clarity western enhanced chemiluminescence blotting substrates (Bio-Rad)
Comparison of ELISA kits Human serum samples were analysed for both isoforms (pro-BDNF and BDNF) in three different brands and at three dilutions per assay, chosen according to: (i) the manufacturers recommendations (Table 1), (ii) the scientific literature (Supplementary Table S1), and (iii) considering one common dilution for all commercial brands tested
Tissue Repair and Musculoskeletal Recovery
