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All PCA ellipse (D) and all no-QC PCA 3D (E)

Control rats (group 1) are fed with standard diet, while the intervention rats are fed with iodine-free diet for 12 weeks to induce hypothyroidism (groups 2-4) which is continued for four more weeks to allow screening of hypothyroid status and L-Thyroxine-treatment

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However, coffee may also contain very small amounts of a chemical called acrylamide, considered a possible carcinogen