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This laccase extract was treated with 0.16 g of PVPP/mL for 10 min and centrifuged at 7500 rpm for 10 min, and the supernatant was subsequently dialysed with 3.5 KDa cellulose membrane for 2 days in a 0.3 M ammonium formate solution and for 2 more days in distilled water

Yes, always practice aseptic technique, including using sterile gloves and disinfecting the injection site

And then well practice

With advancing age, microglia also become primed, responding to inflammatory stimuli with exaggerated cytokine and free radical release [278]

complemented by genetically encoded sensors for compartment-specific, real-time tracking and CRISPRCas systems for GSH-dependent genomic reporting, these integrated platforms collectively facilitate real-time monitoring, precise biomarker detection, and the development of redox-targeted therapies