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Vape pen use may also weaken the lungs' immune defenses and increase the risk of infections with pathogens, including the influenza virus and Streptococcus pneumoniae bacteria, according to a 2022 review published in the journal Cureus

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Methods Materials and reagents The antibodies used in this study were obtained from the following sources: Rabbit anti-ZO-1 (#8193), rabbit anti-claudin-3 (#341700), rabbit anti-VE-cadherin (#D87F2), rabbit anti-VCAM-1 (#12367), mouse PECAM-1 (#89C2) from Cell Signaling Technology (Danvers, MA, USA)