On day 4, the protein expression of key DE markers, SOX17 and forkhead box protein A2 (FOXA2), were evaluated by immunocytochemistry staining on 4% paraformaldehyde (PFA)-fixed cells using SOX17 mouse anti-human monoclonal antibody (Abcam) and FOXA2 rabbit anti-human monoclonal antibody (Abcam), as described below
Feng TY, Li Q, Ren F, Xi HM, Lv DL, Li Y, et al
L5-S1 herniations The L5-S1 level bears the most mechanical stress in the spine, the transition point where the flexible lumbar spine meets the rigid sacrum
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Selenium and Glutathione Peroxidase Function Selenium serves as an essential cofactor for glutathione peroxidase, one of the primary enzymes that utilizes glutathione to neutralize hydrogen peroxide and lipid peroxides