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However, no human clinical studies have tested GHK-Cu for cognitive outcomes

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Cell Culture HepG2 cells were cultured in collagen-coated plates and maintained in DMEM containing 10% FBS, 1% penicillin/streptomycin (PAA Laboratories, Austria), 1% ultraglutamine (Lonza, Switzerland) and 1% non-essential amino acids (Sigma-Aldrich, Germany)

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This is especially the case after the loading period is finished, to help determine the right frequency thereafter