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(100) subjected PS1 gene-knockin mice to continuous EODF for 3 months and found that the EODF group exhibited enhanced resistance to excitotoxic damage in hippocampal CA1 and CA3 neurons, with fewer lipid peroxidation products (4-hydroxy-2-nonenal) compared to the AL group, suggesting that inhibition of oxidative stress might be a potential mechanism for the neuroprotective effects of EODF

cDNA was synthesized by reverse transcription via the LunaScript RT SuperMix Kit (New England Biolabs, Ipswitch, MA, USA)

The data matrices obtained from GC-MS were normalized to the total peak area and transferred to the SIMCA-P+ (v13.0, Umetrics, Umea, Sweden) program for multivariate analysis such as principal component analysis (PCA) and partial least squares differentiation analysis (PLS-DA) for the assessment of separability of the groups

Nucleic Acids Res 43, D345D356, doi: 10.1093/nar/gku1214 (2015)