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By preserving only a Tyr-Ile dipeptide (from angiotensin IV) flanked by hexanoic chains, Harding's laboratory achieved three simultaneous objectives: BBB penetration, stability against aminopeptidases, and oral bioavailability

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Trapped peptides were eluted onto a reversed-phase 25 cm C18 EasySpray nano column (Thermo Fisher Scientific) using a linear gradient of acetonitrile (336%) in 0.1% formic acid over 110 min at 0.3 L/min