However, the negative redox potential of roGFP2, the instantaneous nonenzymatic formation of roGFP2(S 2 ) from roGFP2(SSG), and the very slow nonenzymatic reaction of roGFP2(S 2 ) with GSH make the nonenzymatic shortcut from Fig
The ROS are generated by a number of cellular oxidative and metabolic processes including activity of the enzymes of the mitochondrial respiratory chain, xanthine oxidase, NADPH oxidase, monoamine oxidases, and metabolism of arachidonic acid (AA) mediated by the activity of lipoxygenases (LOX), and ROS are produced primarily by leakage of electrons in the mitochondrial respiratory chain [3]
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