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This could mean multiple physical changes that happen in depression (likely) or it could mean that some of the research is wrong and/or that Im not understanding it correctly (also likely)

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Subsequently, cells were wounded by pipette tips and washed twice with media to remove detached cells, and photomicrographs of initial wounds were taken using Canon PowerShot A640 camera (at 100 magnification)

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Without a preservative, bacteria can proliferate quickly even in trace amounts, leading to cloudy solutions or enzymatic breakdown of the peptide