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ALOX15 KD normalized the intracellular GSH ( P = 0.003), while chemical inhibition of 15LO1 had a marginal, but insignificant effect ( P = 0.066)

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The supernatant was passed through a mouse anti-human AIM IgG monoclonal antibody (CL7)-immobilized HiTrap NHS-activated HP column and the protein was eluted with 0.1 M glycine-HCl (pH 2.5) and neutralized with 1 M Tris-HCl (pH 8.5)