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Contraindications include: Sulfa allergy, due to potential sensitivity to methionine derivatives

For instance, systemic morphine inhibited both the early and late phases of the formalin-induced licking response, and this activity was naloxone-sensitive

Complementary DNA (cDNA) was made by reverse transcription of RNA (2 g) using Moloney murine leukemia virus reverse transcriptase (Promega, Madison, MI, USA) and labeled with Biotin-16-2-deoxy-uridine-5-triphosphate (Roche Diagnostics, Indianapolis, IN, USA)

Once you puncture that vial, its a race against time and contamination