Importantly, we established that ultrafiltered flavan-3,3,4-triol (4 ) was converted to cyanidin ( 8 ) by additional well-characterized arGSTs from maize ( Zm BZ2), arabidopsis ( A tTT19) and grapevine ( Vv GST4), and from the structure-elucidated phi-class orthologue from poplar ( Pt GSTF8), while application of GSTs unrelated to anthocyanin biosynthesis ( Vv GST2, Vv GST5, At GSTF2) did not result in cyanidin ( 8 ) formation (Fig
2c), confirming the observations made with the Nrf2-GFP reporter line
MS/MS ion searches were done against modified cRAP database (based on containing protein contaminants like keratin, trypsin etc., and UniProtKB protein database for Homo sapiens (downloaded 2021-06-16, number of protein sequences: 20,600)
Samocha-Bonet also examined the effect of oral L-glutamine [25 g], whole protein low in glutamine (25 g), or water on the concentration of incretin hormones and insulin response in well-controlled T2DM patients and found that the first-phase insulin response and total GLP-1 were enhanced following the ingestion of both L-glutamine and protein
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