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For experiments involving caspase-3/7 staining, CellEvent Caspase-3/7 Green Detection Reagent (Thermo Scientific C10723) and CPP-CAND were added to live cells 24 h before imaging using an Eclipse Ti-U inverted microscope (NIKON)

For laboratory use, it may be reconstituted with bacteriostatic water or another appropriate solvent according to established research protocols

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GLP-1 agonists are FDA-approved medications with extensive clinical data, while 5-amino-1MQ remains a research compound