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Lim HGY, Fischer AH, Sung S, et al

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10 L of the extracted protein samples were mixed with 3 L of a 5X sample buffer in microcentrifugation tubes, heated to 90 o C for 5 minutes, centrifuged (2,000 x g for 1 minute) and gel electrophoresis took place at 100 Volts for 60 minutes at room temperature