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doi: 10.1080/19490976.2018.1474322 222 WagnerJMaksimovicJFarriesGSimWHBishopRFCameronDJet al

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The following solutions were mixed together: 100 L of cells in TE buffer, 10 L of herring sperm DNA (10 mg mL 1 ), 10 L of SAT1 flipper disruption cassette (0.1 g L 1 ), and 5 L of histamine solution (Qbiogene)