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Results Of the 27 articles selected in the search (Supplementary Figure S1), 2 studies quantified total BDNF, 22 quantified pro-BDNF and 24 quantified mature BDNF in human serum samples

Calcein/PI cell viability/cytotoxicity assay (Live/Dead assay) kit, MTT cell proliferation and cytotoxicity assay kit and crystal violet staining solution were provided by Beyotime Biotechnology (China)