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Human CD34 + cells were cultured in vitro using complete medium (CM) consisting of StemSpan II (StemCell Technologies) medium supplemented with 100 ng l 1 human stem cell factor (R&D systems, 255-SC/CF), 100 ng l 1 human thrombopoietin (R&D systems, 288-TP/CF) and 100 ng l 1 human FLT-3 ligand (R&D systems, 308-FK/CF)