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Description

The 1.077 g/ml density barrier has been widely used since ready-made solutions of this density were previously available as NycoPrep 1.077 and NycoPrep 1.077A, the latter being the more popular for murine samples (see also Section 3)

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Isolation of peripheral blood mononuclear cells (PBMCs) and single-cell suspensions was performed using Ficoll Paque Plus (Millipore Sigma) density gradient centrifugation

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