As indicated by the NAO/TMRE FRET assay, a 12-hour incubation with 20 mM glutamate caused mitochondria membrane potential depolarization which was mitigated by TB, MB, phenothiazine, and 2-chlorophenothiazine with the EC50s of 1.54, 17.72, 21.18, and 45.09 nM respectively
The absorbency was read at 390 nm
The TGF-/NADPH oxidases axis in the regulation of liver cell biology in health and disease
Cis-DDPs well-known nephrotoxicity, indeed, has been proved to be related to his GSH-conjugated compounds that, being metabolized to cysteine S-conjugate by cysteine S-conjugate -lyase activity (highly expressed in renal cells), is mainly responsible for proximal tubule cell damage [114]
The animals were kept in a 12-hour light/dark cycle and maintained on standard rat laboratory chow and tap water ad libitum