Histological analysis and biodistribution For histological analysis, the right knee joint samples were fixed in 4% paraformaldehyde overnight, decalcified in an EDTA solution, and embedded in paraffin blocks
The intrinsic, or mitochondrial, pathway is activated by honeys polyphenolic components that cause mitochondrial membrane depolarization and activate caspases 3, 7, and 9 (84, 85)
In addition, the number of mitochondria increase from primary spermatocytes to round spermatids, a process that requires iron for mitochondrial ironsulfur clusters and heme synthesis
10.1182/bloodadvances.2019000291 181
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