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Replacement of Asn450 by glutamine (N450Q), aspartate (N450D), and alanine (N450A) showed a significant increase in transpeptidase activity up to 3.6-fold enhancement and minor maturational defects, suggesting significant contribution of this residue in substrate binding and catalysis

Improvement in clinical functioning was associated with initial levels of UA and G in the FENNS patients

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The Lancet Psychiatry, 3(8), 760773