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The number of samples was determined by the Universal Population Volume Known Sampling Calculation Formula (n = Nz 2 pq/d 2 (N 1) + z 2 pq)

The anti-folateanti-cancer chemotherapeutic drug methotrexate (Table 1) is used clinically for curing multiple cancers, especially acute leukemia, despite its serious side effects

Similarly, the acetate-producing genus Dubosiella facilitates butyrate synthesis through microbial cross-feeding, as supported by its strong positive correlation with butyrate levels 48,49

Y., Chen, S