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To administer, pinch the skin tightly between your finger and thumb with one hand, and with the syringe in your other hand, inject at a 45-degree angle

Ovarian tissues and whole blood were gathered, with some ovarian tissues being fixed in 4% paraformaldehyde and others stored at -80C

We previously produced the App NL-G-F/NL-G-F -KI ( App NL-G-F ) mouse strain using genomic DNA of introns 15 to 17 of mouse APP, which humanized the A sequence, and introduced KM670/671NL (Swedish), I716F (Iberian), and E693G (Arctic) mutations 41

All procedures were approved by the local ethics committees at the University of So Paulo (Document 45/2009)

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