The advantages of this sensitive technique are that the bimane derivatives have a high fluorescence yield and are quite stable (up to several days in solutions kept in the dark), and that other thiols present in the extract, such as cysteine, can be separated and measured in the same run with glutathione (Figure 2A, top)
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Other forms, including 5-formyl-THF, are converted to 5-methyl-THF by intestinal mucosa (as the organ most responsible for adding the methyl group and reducing the vitamin) or in a small degree by the liver 887,892